ROCHE Universal Protease Substrate

Stock Code: 2795707
Manufacturer Part No: 11734334001

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Descriptions

Application

Use Universal Protease Substrate as a general substrate for proteases, and to detect trace protease activities, for example, as a contamination in enzyme preparations. It can be measured spectrophotometrically and fluorimetrically in a homogeneous assay.

PrincipleBy treatment with proteases, resorufin-labeled peptides are released from resorufin-labeled casein. They cannot be precipitated by trichloroacetic acid. The concentration of these resorufin-labeled peptides in the supernatant is equivalent to the proteolytic activity present.

General description

The Universal Protease Substrate provides rapid and highly sensitive detection of trace protease activity, or determination of the effectiveness of protease inhibition. Protease activity releases resorufin-labeled peptides from the patented substrate, resorufin-labeled casein. Resorufin is N-(resorufin-4-carbonyl)-piperidine-4-carbonic acid).The concentration of these labeled peptides in the supernatant is directly related to the proteolytic activity present. •Conveniently detect nanogram quantities of proteolytic activity in less than one hour. •Perform highly sensitive protease detection in a homogeneous assay.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: Approximately 1 mg/mlStorage conditions (working solution): -15 to -25 °CAn aqueous solution is stable for several months at -15 to -25° C and for 2 to 3 days at 2 to 8° C. It is recommended to store aqueous solutions in aliquots at -15 to -25° C. Repeated freezing and thawing is possible. At 15 to 25 °C the product is rapidly hydrolyzed in solution.

Quality

Function test: performance-controlled in the assay for proteases.