ROCHE PCR Buffer Without MgCl2 10x concentrated

ROCHE PCR Buffer Without MgCl2 10x concentrated

Stock Code: 2795696
Manufacturer Part No: 11699105001
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Application


The PCR buffer without MgCl2 has been used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).


General description


The PCR buffer without MgCl2, in combination with the MgCl2 stock solution, is used for the individual adjustment of the Mg2+ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl2 will yield satisfactory results with a dNTP concentration of 200µM each. In many cases, however, it is required to titrate the optimal Mg2+ concentration to increase the specificity and yield of the PCR reaction.The buffer is ten-fold concentrated and should be used in combination with the MgCl2 stock solution.


Other Notes


For general laboratory use.


Physical form


Solution, 100 mM Tris-HCl, 500 mM KCl (pH 8.3 at 20 °C)

Quality Level100
Storage Temp.−20°C
Formsolution

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