Complete 20 tablets

Complete 20 tablets

Stock Code: 2795694
Manufacturer Part No: 11697498001
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£461.04 (exc VAT) per EACH
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Application


cOmplete™ is used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.cOmplete contains both reversible and irreversible protease inhibitors. Therefore, we recommend the addition of cOmplete to all stock buffers and solutions normally protected with protease inhibitors, and not just during the initial purification steps.cOmplete contains EDTA (1 tablet yields a 1 mM EDTA solution in 50 ml). Therefore, the extraction buffer should not contain divalent cations like Ca2+ , Mg2+, or Mn2+, otherwise the inhibition of the metalloproteases might be incomplete. If the protein of interest will be purified by IMAC (Immobilized Metal Affinity Chromatography), for example, Poly-His-tagged recombinant proteins, EDTA has to be eliminated (e.g., by dialysis) prior to the chromatography.Alternatively, the product cOmplete EDTA-free can be used. These tablets are identical to cOmplete, with the exception that no EDTA or other chelating agent is present.cOmplete Tablets inhibit proteolytic activity in large extraction volumes (up to 50 ml). If you are working with smaller volumes (up to 10 ml), we recommend to use cOmplete, Mini. The composition of the cOmplete, Mini Tablet is identical to the normal cOmplete Tablet.


cOmplete™ Protease Inhibitor Cocktail has been used as a component of lysis buffer for the homogenisation of rat cavernosal smooth muscle (CSM) for obtaining membranous and cytosolic fractions cold buffer A for preparing cytosolic and nuclear extractslysis buffer for homogenisation of tissues for determining protein expression by western blot phosphate buffered saline (PBS) for the lysis of rat retinae for enzyme linked immunosorbent assay (ELISA)


Components


Proprietary mixture of several protease inhibitors with broad inhibitory specificity.


Features and Benefits


Use cOmplete Protease Inhibitor Tablets to protect your proteins from a wide range of proteases. In just minutes, uninhibited proteolytic activity can degrade the protein you have spent days isolating. Each of these convenient water-soluble tablets contains a blend of protease inhibitors that inhibits proteolytic activity from most cell types, including animals, plants, yeast, and bacteria. No weighing or measuring is necessary, ensuring consistent results. Easy-to-use: Simply drop a quick-dissolving tablet into your buffer. Complete protection: Instantly protect your proteins against a broad range of proteases. Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi. Safe: Choose non-toxic inhibitors that pose no risk to you or those around you. Suitable for IMAC purification: Purify poly-histidine tagged proteins with cOmplete His-Tag Purification Resin.


General description


cOmplete Protease Inhibitor Tablets inhibit a broad spectrum of serine, cysteine, and metalloproteases, as well as calpains. Due to the optimized composition of the tablets, they show excellent inhibition effects, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria

Storage Temp.2-8°C
Solubilityaqueous buffer: soluble
Formtablet

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