ROCHE Pwo DNA Polymerase

ROCHE Pwo DNA Polymerase

Stock Code: 2795664
Manufacturer Part No: 11644955001
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£632.36 (exc VAT) per EACH
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Application


Due to its proofreading activity, the thermostable Pwo DNA Polymerase has an extremely low error rate, 18-fold lower compared to Taq DNA Polymerase. It is therefore ideal for applications that require the highest possible fidelity in DNA synthesis. It can be applied for High fidelity PCRCloning of PCR productsCharacterization of rare mutationsPCR


Features and Benefits


Excellent accuracy (18-fold more accurate than Taq DNA polymerase) High thermal stability Nearly as processive as Taq DNA polymerase Accepts modified nucleotides


General description


Pwo DNA Polymerase is a highly processive thermostable 5?-3? DNA polymerase and possesses a 3?–5? exonuclease activity, also known as proofreading activity. The enzyme has no detectable 5?–3? exonuclease activity. Pwo DNA Polymerase exhibits increased thermal stability with a half-life of more than two hours at +100 °C, compared to Taq DNA Polymerase?s half-life of less than 5 minutes at this temperature. Pwo DNA Polymerase-generated PCR products are blunt-ended and can therefore be used directly for blunt-end ligation without any pretreatment of the ends.


Legal Information


Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.﹤/ br>NOTICE TO PURCHASER: LIMITED LICENSE﹤br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany

Quality Level100
Technique(s)PCR: suitable
Featurehotstart: no, High Fidelity PCR, dNTPs included: no
Storage Temp.−20°C

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