ROCHE PCR Master

ROCHE PCR Master

Stock Code: 2795661
Manufacturer Part No: 11636103001
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£470.79 (exc VAT) per EACH
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Application


The PCR Master can be used instead of the single reagent Taq DNA Polmerase, or instead of the PCR Core Kit for nearly all simple, routine polymerase chain reaction applications. The only limitation is that the sample volume must not exceed half the total reaction volume. The PCR master mix is used for:Routine PCRRT-PCROther primer-extension reactions, such as sequencing and labeling


Features and Benefits


Reliable reproducible results:High lot-to-lot consistency. Convenience:Adding template and primer requires only two pipetting steps. Prevent PCR carryover:dUTP incorporation combination with Uracil-DNA Glycosylase prevents PCR cross-contamination. Ready for immediate use:Master mix can be stored at +2 to +8°C.


General description


The PCR Master contains all the reagents required to perform a standard PCR. All that must be added is the template DNA, the primers, and the required amount of water. The PCR Master contains a fixed MgCl2 concentration of 1.5 mM. However, higher concentrations may be achieved by adding additional MgCl2. PCR products generated by PCR Master are 3′-A single-overhang products. Therefore T/A cloning can be applied.The PCR Master is stable at +2 to +8 °C, allowing immediate PCR without the time-consuming thawing of reagents.


Routine assays have medium size amplicons and 50% GC content. Taq DNA Polymerase has no proofreading or hot start features. It is optimally active at +75°C and pH 9. The 5′ → 3′ DNA polymerase lacks 3′ → 5′ exonuclease activity. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 95 kD.Lack of restriction endonuclease:The enzyme originally isolated from T. aquaticus BM lacks Taq I restriction endonuclease activity.


Legal Information


Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.﹤/ br>NOTICE TO PURCHASER: LIMITED LICENSE﹤br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research

Quality Level100
Technique(s)PCR: suitable
Featurehotstart: no, dNTPs included
Storage Temp.−20°C

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