ROCHE hGH ELISA

ROCHE hGH ELISA

Stock Code: 2795655
Manufacturer Part No: 11585878001
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Analysis Note


No cross-reaction with rat growth hormone. Cross reactivity with the human homologues TSH, FSH, and LH has not been tested.


Application


For research use only. Not for use in diagnostic procedures.The hGH ELISA is used to quantitatively measure the expression level of hGH released into cell culture supernatant of eukaryotic cells transfected with a plasmid containing a reporter gene that encodes for hGH.


Features and Benefits


Standardized: Allows direct comparison of data from different sets of experiments, even when kits from different production lots are used More sensitive: The hGH ELISA is approximately 20-times more sensitive than isotopic hGH assays More specific: The hGH ELISA shows no crossreactivity with rat growth hormone Faster: Measures hGH expression as early as 18hours post-transfection and requires only approximately 4 hours from start to finish Easy to perform: Follows a standard ELISA protocol


General description


The hGH assay system differs from most of the other commonly used reporter proteins (CAT, β-Gal, or luciferase) in one important respect: hGH is a secreted protein, and is measured using samples of culture-medium supernatants thus (i) avoiding the necessity to lyse cells, (ii) allowing continuous monitoring of transient expression kinetics, and (iii) allowing use of the cells for other purposes (e.g., RNA isolation). Additionally, hGH is a mammalian gene that may contribute to the apparent high stability of the hGH mRNA in most mammalian cells.Using the standard isotopic protocol, secretion of hGH protein into the culture-medium supernatant can be monitored approximately 24hours post-transfection, depending on the cell type and the sensitivity of the assay used. Usually, hGH is quantified by an immunoradiometric assay (IRMA), resulting in a linear range of approximately 0.1 to 50ng hGH/ml.


Human growth hormone (hGH) is specifically detected by the hGH ELISA. It does not cross-react with rat growth hormone. Cross-reactivity with the human homologues TSH, FSH, and LH has not been tested. The hGH protein from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.hGH StandardsThe hGH protein from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.


Other Notes


For life science research only. Not for use in diagnostic procedures.


Packaging


1 kit containing 9 components.


Preparation Note


Working solution: Solution 1 hGH Stock Solution (bottle 1)Reconstitute the lyophilizate in 0.5 ml double distilled water. The resulting concentration is calculated using the lot-specific information, given on the label (final conc. approx 10 ng/ml). This solution is used for the preparation of hGH standards for establishing a hGH calibration curve (see 7.2: preparation of hGH standards).Solution 2 Anti-hGH-DIG (bottle 2)Reconstitute the lyophilizate in 0.5 ml double distilled water (final conc. 100 µg/ml).

Quality Level100
Technique(s)ELISA: suitable
Storage Temp.2-8°C
Assay>98% (SDS-PAGE)

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