ROCHE Streptavidin-AP-conjugate for nucleic acid detection

ROCHE Streptavidin-AP-conjugate for nucleic acid detection

Stock Code: 2795529
Manufacturer Part No: 11089161001
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Application


Streptavidin-AP has been used to detect biotinylated compounds using:ELISAImmunohistocytochemistryWestern blot


General description


Streptavidin is covalently coupled to alkaline phosphatase (AP) for the preparation of streptavidin-AP-conjugate.Alkaline PhosphataseAlkaline Phosphatase is often used as a label in enzyme immunoassays. AP converts the sensitive substrate X-phosphate/NBT into a stable, water soluble product.


Other Notes


For life science research only. Not for use in diagnostic procedures.


Physical form


Solution, stablilized in 50 mM Triethanolamine buffer, 3 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2, 10 mg/ml bovine serum albumin, pH 7.6.


Preparation Note


Working solution: 50 - 1,000 mU/ml (i.e., stock solution can be diluted). Dilute the conjugate stock solution in 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.Working concentration: Working concentration of conjugate will depend on the application and substrate. Indicated concentrations should be taken as a guideline.ELISA: 50 - 200 mU/ml (1:5000 - 1:20,000 dilution)Immunohisto/cytochemistry: 250 - 1,000 mU/ml (1:1000 - 1:4000 dilution)Western blot: 200 - 500 mU/ml (1:2000 - 1:5000 dilution)Stability: The concentrated stock solution is stable when stored at 2 to 8°C until the expiration date printed on the label. Do not freeze!

Quality Level100
Storage Temp.2-8°C
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