HP RNA PARAFFIN KIT

Stock Code: 2795226
Manufacturer Part No: 3270289001

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Descriptions

Analysis Note

Nucleic acids bind to the surface of the glass fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure Filter Tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants. For RNA isolation, the binding conditions can be optimized to ensure immobilization of all the RNA.Capacity: The High Pure Spin Filter Tubes hold up to 800 µL sample volume.Sample Material: 5 - 10 µm sections from formalin-fixed, paraffin-embedded tissue (e.g., colon, breast, liver, kidney, spleen of mammalian species, including human-research samples). 20 – 30 mg fresh-frozen solid tissue. 3 × 5 µm tissue sections from fresh-frozen tissue.

Application

The High Pure RNA Paraffin Kit isolates total RNA from paraffin embedded, fresh-frozen tissue. The quality of RNA obtained is suitable for relative quantification of mRNA with RT-PCR, especially on the Lightcycler^® Carousel-Based System. Additional applications include: RT-PCR Differential display RT-PCR cDNA synthesis Primer extension

Components

Tissue Lysis Buffer Proteinase K, PCR-grade Binding Buffer Wash Buffer I Wash Buffer II DNase I DNase Incubation Buffer Elution Buffer High Pure Filter Tubes Collection Tubes

Features and Benefits

The High Pure RNA Paraffin Kit isolates total RNA from formalin-fixed, paraffin-embedded tissue as well as from fresh-frozen tissue-research samples for direct use in RT-PCR. Isolate RNA suitable for RT-PCR from archived paraffin tissue (up to 15 years old). Obtain a concentrated product that is ready-to-use (no RNA precipitation required). Quickly prepare total RNA from tissue sections in approximately 2 hours (after an overnight digestion). Obtain highly pure RNA for use in relative mRNA quantification procedures (e.g ., with the Lightcycler^® System). Eliminate the use of hazardous organic compounds such as cesium chloride, phenol, chloroform, and ethidium bromide.

General description

Low to medium throughput RNA isolation from FFPE sample materials.

Legal Information

LightCycler is a registered trademark of Roche

Other Notes

For life science research only. Not for use in diagnostic procedures.

Purified RNA is free of DNA, nucleases, and all cellular and sample components that interfere with RT-PCR. Typically, RNA fragments isolated from formalin-fixed tissue ranged from 150 up to 1500 bases. The bulk of RNA is 250 bases in length.

High Pure Technology and Silica Adsorption Kits

Preparation Note

Tissue samples are disrupted and homogenized during an overnight incubation with Proteinase K (paraffin samples) or by using a suitable tissue homogenizer (fresh-frozen tissue). Nucleic acids bind in the presence of a chaotropic salt specifically to the surface of the glass fibers pre-packed in the High Pure Purification Filter Tube (1). The binding reaction occurs within seconds due to the disruption of the organized structure of water molecules and the interaction with nucleic acids