Ready-To-Go You-Prime First-Strand Beads

Stock Code: 1669890
Manufacturer Part No: 27926401

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Descriptions

The kit provides 50 preformulated, single-dose reaction beads prepackaged in thin-walled 0.5 ml tubes compatible with most thermal cyclers. For synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice.

For synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice for RT-PCR.
Predispensed, ambient-temperature-stable, single-dose reaction beads minimize pipetting errors, avoid cross-contamination, and ensure optimal performance.
First-strand reaction beads contain no primer, thus allowing users to select a first-strand primer of choice.
Highly suitable for research applications that use PCR to detect and quantify eukaryotic RNA from a variety of samples.
Reaction beads are function-tested for first-strand synthesis of cDNA up to 7.5kb and in RT-PCR from blood samples.
Completed reactions (33µL final volume) can be used directly in PCR after adding water, Taq DNA polymerase, and primers; first-strand cDNA can also be used as a template for traditional Gubler-Hoffman second-strand cDNA synthesis.