Neomycin trisulfate salt hydrate, plant cell culture tested

Neomycin trisulfate salt hydrate, plant cell culture tested

Stock Code: 3582060
Manufacturer Part No: N3144-100G
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Application


Neomycin Trisulfate is produced by Streptomyces containing a minimum of 85% neomycin B. It has been used as a selection agent for prokaryotic cells transformed using the neo selectable marker gene, and to study ototoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts, and extraction of Nuclear Phosphatidylinositol 4,5-Bisphosphate-Interacting Proteins. It is recommended for use in cell culture applications at 50 mg/L.


Biochem/physiol Actions


Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca2+ channels without affecting the Na+/Ca2+ antiporter in neurons.Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria.


Caution


Stock solutions should be filter sterilized and stored at 2-8°C, and are stable at 37°C for 5 days. A 1 mg/mL neomycin solution in .1 M phosphate buffer, pH 8.0 should be used within 14 days when stored at 0-5°C. Solutions should be protected from light or moisture.


General description


Chemical structure: aminoglycoside


Packaging


100 g in glass bottle


Preparation Note


Neomycin sulfate is soluble in H2O at 50 mg/mL, yielding a clear solution.

Quality Level200
InChI keyWHAGUNPVKDUVFV-QGTTWHFQSA-N
InChI1S/C23H46N6O13.3H2O4S.H2O/c24-2-7-13(32)15(34)10(28)21(37-7)40-18-6(27)1-5(26)12(31)20(18)42-23-17(36)19(9(4-30)39-23)41-22-11(29)16(35)14(33)8(3-25)38-22;3*1-
ManufacturerSIGMA-ALDRICH
Formpowder

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