Murashige and Skoog Vitamin Solution, 1000 x, liquid, plant cell culture tested

Murashige and Skoog Vitamin Solution, 1000 x, liquid, plant cell culture tested

Stock Code: 3582007
Manufacturer Part No: M3900-50ML
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Application


Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).Use at a concentration of one ml per liter of prepared medium to achieve the proper final concentration.


Components


Solution contains (mg/ml): 2.0 glycine, 100.0 myo-inositol, 0.50 nicotinic acid, 0.50 pyridoxine hydrochloride, 0.10 thiamine hydrochloride.


Preparation Note


Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Vitamin powder (M3900) can be combined with Murashige and Skoog micro/macro-nutrients to generate basal media which when supplemented with organics, agar, auxins and cytokinins yield complete medium for growth plant tissue culture. The M3900 Solution is a 1000x concentrate which contains (mg/ml): 2.0 glycine, 100.0 myo-inositol, 0.50 nicotinic acid, 0.50 pyridoxine hydrochloride, 0.10 thiamine hydrochloride.

Colourcolorless
Quality Level400
pH5.5-7.5
ManufacturerSIGMA-ALDRICH
Formliquid

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