SANGER NON-TARGET CONTROL#2, DNA

SANGER NON-TARGET CONTROL#2, DNA

Stock Code: 3581868
Manufacturer Part No: CRISPR19-1EA
Order Now for 7 day delivery
£303.64 (exc VAT) per EACH
Quantity: - +

Application


Functional Genomics/Screening/Target Validation


General description


The Lenti CRISPR Non-Target Control#2 (Lv04 vector) is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. The DNA format of this control construct permits direct transfection in knockout experiments or use in the viral packaging process (for lentiviral-based knockdown experiments). This vector is useful as a negative control in experiments using CRISPR lentiviral clones. The lenti CRISPR non-target control uses a dual-component system consisting of U6-driven guide RNA (non-targeting) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.


Legal Information


CRISPR Use License AgreementLentiviral License Agreement


Other Notes


Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP002).

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formliquid

There are no downloads for this product.