Casein Blocking Buffer, powder for blocking hybridization blots

Casein Blocking Buffer, powder for blocking hybridization blots

Stock Code: 3581861
Manufacturer Part No: C7594-1L
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£182.46 (exc VAT) per 1L
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Application


Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.


Features and Benefits


Better blocking performance than dried milk or BSA Compatible with neutral and positively-charged membranes Compatible with chemiluminescent, colorimetric , and DIG detection assays


General description


Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.


Packaging


1 L in poly bottle


Preparation Note


Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.

Quality Level200
pH6.8-7.2(reconstituted per directions)
ManufacturerSIGMA-ALDRICH
Storage Temp.room temp
SolubilityH2O: soluble, faintly yellow (dissolves in 5-10 minutes.)
Formpowder blend

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