Poly(2-deoxyinosinic-2-deoxycytidylic acid) sodium salt, free from low molecular weight nucleotides,

Stock Code: 3581775
Manufacturer Part No: 81349-500UG

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Descriptions

Application

Suitable for non-specific blocking agent in EMSA evaluation of DNA methyltransferases DNA-small molecule or drug interaction studies

Poly(2?-deoxyinosinic-2?-deoxycytidylic acid) sodium salt has been used in electrophoretic mobility shift assay (EMSA) of the transcription factor AP-2 Alpha (TFAP2A) and in southwestern blotting of human embryonic kidney cells (HEK293) nuclear extract.

Biochem/physiol Actions

Poly(2?-deoxyinosinic-2?-deoxycytidylic acid), poly(dI-dC) is a synthetic DNA substrate. At high salt, Poly(dI-dC) exists in left handed helical conformation and reverts to right-handed form upon decreasing salt. It is used as a non-specific competitor in electrophoretic mobility shift assay (EMSA).

General description

Poly (2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt is an alternating copolymer that acts as a substrate for DNA methyltransferases.