Medium 199, HEPES Modification, with Earles salts, L-glutamine and 25 mM HEPES, without sodium bicar

Medium 199, HEPES Modification, with Earles salts, L-glutamine and 25 mM HEPES, without sodium bicar

Stock Code: 3586170
Manufacturer Part No: M2520-10X1L
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General description


Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.


Quantity


Formulated to contain 14.7 grams of powder per liter of medium.


Reconstitution


Supplement with 2.2 g/L sodium bicarbonate.

Quality Level500
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - mammalian: suitable
Storage Temp.2-8°C
Formpowder

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