Nutrient Mixture F-12 Ham, powder, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bica

Nutrient Mixture F-12 Ham, powder, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bica

Stock Code: 3586134
Manufacturer Part No: F6636-10X1L
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Application


Coon's modification of Ham's F-12 was developed for culturing hybrid cells that were produced by viral fusion. The modification consists of doubling the amino acids and pyruvate and including ascorbic acid. The salt concentrations have also been altered. The formula contains 0.863 mg/L zinc sulfate, which may render it unsuitable for culturing mouse L-cells.


Quantity


Formulated to contain 11.5 grams of powder per liter of medium.


Reconstitution


Supplement with 2.68 g/L sodium bicarbonate.

ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - hybridoma: suitable, cell culture - mammalian: suitable
Storage Temp.2-8°C
Formpowder

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