GSEM Supplement, 50 x, liquid, sterile-filtered, suitable for cell culture

Stock Code: 3577072
Manufacturer Part No: G9785-100ML
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Application


GSEM Supplement has been used as a supplement for ProCHO5 Protein-free medium for culturing Chinese hamster ovary (CHO) cells and selective medium for NS0 myeloma cells.


General description


Glutamine synthetase expression medium (GSEM) supplement enables formation of glutamine from ammonia and glutamate there by alleviating accumulation of ammonia in culture medium. It is exploited for high yield recombinant protein production in Chinese hamster ovary (CHO) cells.


Packaging


Contains (mg/L): L-alanine 450.0, L-asparagine · H2O 4261.0, L-aspartic acid 650.0, L-glutamic acid 3750.0, L-proline 575.0, L-serine 500.0, adenosine 350.0, guanosine 350.0, cytidine 350.0, uridine 350.0, and thymidine 12.0


Quantity


One mL will supplement 50 mL of cell culture medium.

Quality Level200
Pack100ML
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - mammalian: suitable
Storage Temp.−20°C
Impuritiesendotoxin, tested
Formliquid

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