Dulbeccos Modified Eagles Medium/Nutrient Mixture F-12 Ham, With L-glutamine and sodium bicarbonate,

Stock Code: 3577053
Manufacturer Part No: D8062-500ML
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Application


Dulbecco?s Modified Eagle?s Medium/Nutrient Mixture F-12 Ham has been used:to maintain human lung and colorectal cancer cell lines for the biological evaluation of the secondary metabolitesto culture human kidney-2 (HK-2) cells and as a component of the assay medium for lysophosphatidylcholine (LPC) treatmentto culture mouse squamous cell carcinoma (SCCVII) tumor cells


General description


Media are supplemented with a defined mixture of nutrients, growth factors, and hormones instead of serum. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used for these defined studies. To compensate for the loss of buffering capacity caused by eliminating serum, a final concentration of 15 mM HEPES buffer can be added to the formulations. It allows the investigator to optimize the buffering system.

Quality Level300, 400
Pack500ML
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - mammalian: suitable
Storage Temp.2-8°C
Impuritiesendotoxin, tested
Formliquid

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