Cathepsin F Active human, recombinant, expressed in FreeStyle 293-F cells, >=90% (SDS-PAGE)

Stock Code: 3581380
Manufacturer Part No: SRP0290-50UG

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Descriptions

Application

Active human cathepsin F is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human cathepsin F has been used in a study to assess its implications for the development of novel immunomodulators. Active human cathepsin F has also been used in a study to investigate microarray expression analysis and identification of serum biomarkers for Niemann-Pick disease.

General description

Human cathepsin F (GenBank Accession No. NM_003793), CD33 signal peptide(amino acids 1-16) + cathepsin S (amino acids 20-end), with C-terminal HIS tag, MW = 53 kDa (more than 58 kDa after post-modification), expressed in FreeStyle 293-F cells.

Legal Information

FreeStyle is a trademark of Invitrogen Corp.

Physical form

Formulated in 45 mM sodium acetate, 135 mM NaCl and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C