Phosphatase substrate, 40 mg tablets

Phosphatase substrate, 40 mg tablets

Stock Code: 3581130
Manufacturer Part No: P5994-25TAB
Order Now for 21 day delivery
Click Add to Quote for price and delivery
Quantity: - +

Application


Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.


The substrate has been used to determine the alkaline phosphatase (ALP) activity in human osteoblast-like Saos-2 cell lysates. ALP acts as an early marker of osteoblast differentiation that is expressed just before matrix mineralization begins.


Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.


Phosphatase substrate has been used as a substrate in alkaline phosphatase assay in serum samples, bone marrow stromal cells and human mesenchymal stem cells (hMSC).


General description


Human phosphatase-substrate networks are mapped based on the literature and protein-protein interaction (PPI) database. Based on gene expression data, the possible network of phosphatase-substrate-kinase is predicted.


Other Notes


Substrate for the determination of acid and alkaline phosphatases.


Packaging


25 tablets in glass bottle


Preparation Note


Dissolve tablets to the desired concentration in either of the following buffers: 0.1 M glycine, pH 10.4, with 1 mM MgCl2 and 1 mM ZnCl2 or 1 M diethanolamine, pH 9.8, with 0.5 mM MgCl2. Typically, a pNPP concentration of 1 mg/mL is used.


Quantity


Contains 40 mg substrate per tablet

Quality Level200
InChI keyKAKKHKRHCKCAGH-UHFFFAOYSA-L
InChI1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
ManufacturerSIGMA-ALDRICH
Impuritiesp-nitrophenol, essentially free
SolubilityH2O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.)
Formtablet

There are no downloads for this product.