Phosphatase, Alkaline from porcine kidney, lyophilized powder, 100-300 DEA units/mg protein

Phosphatase, Alkaline from porcine kidney, lyophilized powder, 100-300 DEA units/mg protein

Stock Code: 3581072
Manufacturer Part No: P4439-100UN
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Analysis Note


Protein determined by biuret.


Application


Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling.


Biochem/physiol Actions


Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids.


Packaging


Package size based on DEA units


100 units in serum bottle


Physical form


Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0


Unit Definition


One unit will hydrolyze 1.0 µmole of p-nitrophenyl phosphate per min at pH 9.8 at 37?°C.

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, ≥50%
Storage Temp.−20°C
Formlyophilized powder

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