Neuraminidase from Vibrio cholerae, Type II, buffered aqueous solution, 8-24 units/mg protein (Lowry

Stock Code: 3579936
Manufacturer Part No: N6514-1UN
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Application


Neuraminidase from Vibrio cholera has been used in a study to describe a five-step purification method. It has also been used in a study to investigate modification of leukemia L1210 tumor cells.


Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.


General description


Calorimetry studies show that the two lectin-like domains flanking the central catalytic domain of Neuraminidase serve as the recognition and binding sites for sialic acid-containing substrates.


Neuraminidase enzymes are hydrolase enzymes that promote influenza virus release from infected cells and facilitate virus spread.


Physical form


Solution in 50 mM sodium acetate, pH 5.5, containing 0.15 M sodium chloride and 4 mM calcium chloride, 0.2 µm filtered..


Preparation Note


A further purification by affinity chromatography of our Type III.


Quality


Preservative free.


Unit Definition


One unit will liberate 1.0 µmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formbuffered aqueous solution

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