Glycerol 3-phosphate Oxidase from Pediococcus sp., lyophilized powder, 40-80 units/mg solid, pH 8.1

Glycerol 3-phosphate Oxidase from Pediococcus sp., lyophilized powder, 40-80 units/mg solid, pH 8.1

Stock Code: 3579662
Manufacturer Part No: G9637-100UN
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Application


Glycerol 3-phosphate Oxidase from Pediococcus sp. has been used in the kinetic assay to study gluconeogenesis in liver and in immobilization studies on pencil graphite (PG) electrode.


This enzyme is useful for enzymatic determination of triglyceride when coupled with lipoprotein lipase (LPL-311, LPL-314) and glycerokinase (GYK-301, GYK-311) in clinical analysis.


General description


Glycerol 3-phosphate Oxidase is a FAD-dependent ?-glycerophosphate oxidase and catalyzes the formation of dihydroxyacetonephosphate. Glycerol 3-phosphate oxidase comprises of a flavin adenine dinucleotide (FAD) and a substrate binding domain and corresponds to molecular weight of 42 kDa. It exists as a monomer and the active site comprises of isoalloxazine ring and histidine 53, arginine 316 and lysine 345 residues crucial for ligand binding.


Packaging


100 units in glass bottle


Physical form


Lyophilized powder containing stabilizers


Physical properties


Isoelectric point : 4.1 -/+0.1Michaelis constants : 3.2x10 -3M (L-α-Glycerophosphate), 6.8 x 10 -3M (D, L-form)Inhibitors : Ionic detergents (SDS, LBS, etc.), Hg++, Ag+Optimum pH : 8.0 - 8.5Optimum temperature : 35 - 40oC pH Stability : pH 6.5 - 8.5 (25oC, 20hr) Thermal stability : below 40oC (pH 7.0, 15min) Substrate specificity : The enzyme has the highest specificity for L-form of a-γlycerophosphate


Unit Definition


One unit will oxidize 1.0 µmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H2O2 per min at 37°C, at the appropriate pH.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formlyophilized powder

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