Endo-beta-galactosidase from Bacteroides fragilis, recombinant, expressed in E. coli, >=140 units/mg

Stock Code: 3579596
Manufacturer Part No: G6920-.5UN

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Descriptions

Application

Endo-?-galactosidase was used in fractional protein isolation. It was used for deglycosylation in glycoproteomics of the endothelial secretome of human endothelial cells.

Biochem/physiol Actions

Internal ?(1-4) galactose linkages in unbranched, repeating poly-Nacetyllactosamine [GlcNAc ?(1-3)Gal ? (1-4)] structures are the preferred substrate.Sulfated structures such as keratan sulfate are also cleaved. Branching and/or fucosylation of the substrate may reduce or completely inhibit cleavage. Sulfation of C-6 on galactose will block cleavage. Oligosaccharides of the neolacto-group are cleaved at greatly reduced rates depending on the deviation from the preferred substrate. For example, Gal ?(1-3)GlcNAc ?(1-3) Gal ?(1-4)Glc is cleaved at 5X10^-5 the rate of keratan sulfate?-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of ?-galactosidase, in a feedback loop.

Packaging

0.5 unit in glass insert

Physical form

Aseptically filled solution in 20 mM Tris-HCl, pH 7.5

Unit Definition

One unit will release 1.0 µmole of reducing sugar from bovine corneal keratan sulfate per minute at 37 °C, pH 5.8.