Glycerokinase from Cellulomonas sp., lyophilized powder, 25-75 units/mg protein

Stock Code: 3579581
Manufacturer Part No: G6142-1KU

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Descriptions

Application

This enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerol-3-phosphate dehydrogenase (=G-3-P DH, G3D-301), glycerol-3-phosphate oxidase (=G-3-P oxidase, G3O-301, G3O-311, G3O-321) or pyruvate kinase (PYK-301) and lactate dehydrogenase (LCD-209, LCD-211), lipoprotein lipase (LPL-311, LPL-314) in clinical analysis.

Biochem/physiol Actions

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.

Packaging

1000 units in poly bottle

Physical form

Lyophilized powder containing phosphate buffer salts and sodium gluconate

Physical properties

Isoelectric point : 4.2Michaelis constants : 4.4 x 10^-5M (Glycerol), 4.3 x 10^-4M (ATP)Inhibitors : p-Chloromercuribenzoate, heavy metal ions (Pb⁺⁺, Fe⁺⁺, Hg⁺⁺, Ag⁺)Optimum pH : 9.8 (G-3-PDH system), 7.8 (G-3-P oxidase system) Optimum temperature : 500^C pH Stability : pH 5.5 x 10.0 (25o^C, 20hr) Thermal stability : below 40o^C (pH 7.5, 15min) Substrate specificity : This enzyme catalyzes the stereospecific transfer of the terminalphosphoryl moiety of ATP to one of the primary hydroxyl group ofglycerol, forming sn-glycerol-3-P. The enzyme has the highestspecificity for glycerol, and also phosphorylates dihydroxyacetoneand glyceraldehyde (Table 1,2). Mg+⁺is essentially required for thereaction.

Unit Definition

One unit will convert 1.0 µmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.