Glycerokinase from Escherichia coli, lyophilized powder, 50-100 units/mg protein

Stock Code: 3579546
Manufacturer Part No: G4509-1KU
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Application


Glycerol kinase (glpK) was used to study the effects of pain controlling neuropeptides on human fat cell lipolysis.


Biochem/physiol Actions


Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. ﹤﹤﹤20,21>>> It is also subject to feedback regulation by fructose-1,6-bisphosphate.﹤﹤﹤22>>>


Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.


Packaging


1000 units in serum bottle


Physical form


Partially purified lyophilized powder, balance is primarily salts and EDTA.


Unit Definition


One unit will convert 1.0 µmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, ≥50% biuret
Storage Temp.−20°C
Formlyophilized powder

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