Furin human, >=2,000 unit/mL, buffered aqueous solution, recombinant, expressed in baculovirus infec

Furin human, >=2,000 unit/mL, buffered aqueous solution, recombinant, expressed in baculovirus infec

Stock Code: 3579439
Manufacturer Part No: F2677-50UN
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Application


Furin is capable of cleaving precursors of a wide variety of proteins, including growth factors, serum proteins, including proteases of the blood-clotting and complement systems, matrix metalloproteinases, receptors, viral-envelope glycoproteins, and bacterial exotoxins, typically at sites marked by the consensus sequence Arg-Xaa-(Lys/Arg)-Arg.


Biochem/physiol Actions


Furin is a dibasic endoprotease that is localized in the Golgi apparatus. It has a molecular mass of 52.7 kDa. It is responsible for the proteolytic maturation of many precursor proteins in the secretory and endocytic pathways of mammalian cells. Furin cleaves precursor proteins at their paired basic amino acid processing sites. Some substrates of furin include von Willebrand factor, transforming growth factor beta 1 precursor, pro-beta-secretase and proparathyroid hormone.


Furin is a dibasic endoprotease that is localized in the Golgi apparatus. It is responsible for the proteolytic maturation of many precursor proteins in the secretory and endocytic pathways of mammalian cells.


Physical form


Solution in 10 mM MES, pH 7.0 at 25 °C, 1 mM CaCl2, 50% glycerol.


Preparation Note


Isolated from Spodoptera frugiperda (Sf9) cells infected with recombinant baculovirus carrying truncated human furin


Unit Definition


One unit is defined as the amount of enzyme required to cleave 25 µg of a MBP-FN-paramyosin-δSal substrate to 95% completion in 6 hours at 25°C in a total reaction volume of 25 µl.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−70°C
Formbuffered aqueous solution

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