Chondroitinase AC from Flavobacterium heparinum, recombinant, expressed in E. coli, >=200 units/mg p

Stock Code: 3579402
Manufacturer Part No: E2039-10UN

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Descriptions

Application

Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.

Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).

Highly purified to remove interferring ?-glucuronidase and protease activities for use in the hydrolysis of chondroitin sulfate pror to HPLC analysis. ?-Glucuronidase activity: ?2,000 femtomole/min/mg protein, Protease activity: ?2,000 femtomole/min/mg protein

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Unit Definition

1 unit is defined as the amount of enzyme that will liberate 1.0 µmole per minute of unsaturated disaccharides from chondroitin sulfate A at pH 6.7 at 37 °C as measured by the change in A232. The ε^µµ for the reaction product δ-Di-4S (chondroitin sulfates A and B) is 5.1 and 5.5 for δ-Di-6S (chondroitin sulfate C).