Dextran Sucrase from Leuconostoc mesenteroides, lyophilized powder, >=100 units/mg protein

Dextran Sucrase from Leuconostoc mesenteroides, lyophilized powder, >=100 units/mg protein

Stock Code: 3579375
Manufacturer Part No: D9909-10UN
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Application


The enzyme from Sigma has been used to prepare immobilized sphere for the production of dextran from sucrose.


Dextran sucrase from Leuconostoc mesenteroides has been used in a study to investigate the functional and structural characterization of α-(1→2) branching sucrase derived from DSR-E glucansucrase. Dextran sucrase from Leuconostoc mesenteroides has also been used in a study to investigate the bioengineering of Leuconostoc mesenteroides glucansucrases.


Dextran Sucrase from Leuconostoc mesenteroides has been used:in immobilization on shirasu porous membrane (SPG) for dextran productionin the enzymatic synthesis of dextran nanoparticles at various pH rangein the immobilization with hydroxyapatite for dextran production


Biochem/physiol Actions


Dextransucrases are glucansucrases that are able to produce dextran, a glucose polymer linked mainly through ?1-6 bonds. However, ?1-3, ?1-6, ?1-4 and ?1-2 bonds are also found, in both the main chain and the branching linkages. The peptide has approximately 1600 amino acids. The aspartic acid in position 551 is essential for catalytic activity, while glutamic acid 589 and aspartic acid 662 complement the catalytic triad. The activity of dextransucrase is decreased by EDTA, and is restored by the addition of calcium ions. Zinc, cadmium, lead, mercury and copper ions are inhibitory to various degrees.


General description


Dextran Sucrase from Leuconostoc mesenteroides belongs to glycoside hydrolase family 70 (GH70). It functions through a retaining mechanism and uses two catalytic acidic residues. Dextran sucrase has a dextran binding site in the C-terminal domain.


Packaging


10 units in glass bottle


Physical form


Lyophilized powder containing dextran, MES buffer salts and CaCl2


Quality


Chromatographically purified


Unit Definition


One unit will liberate 1.0 µmole of fructose per min at 37 °C, pH 5.4.

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, ~15% Lowry
Storage Temp.−20°C
SolubilityH2O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow
Formlyophilized powder

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