Chondroitinase AC from Flavobacterium heparinum, lyophilized powder, 0.5-1.5 units/mg solid (using c

Chondroitinase AC from Flavobacterium heparinum, lyophilized powder, 0.5-1.5 units/mg solid (using c

Stock Code: 3579149
Manufacturer Part No: C2780-5UN
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Application


Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.


Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.


Biochem/physiol Actions


Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).


Other Notes


View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer


Contains potassium phosphate buffer salts and BSA as stabilizer.


Packaging


5 units in serum bottle


Preparation Note


Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.


Suitability


Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides


Unit Definition


One unit will cause a δA232 of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm).

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, ~15% Lowry
Storage Temp.−20°C
Solubility0.02 M phosphate buffer: soluble (pH 7.0)
Formlyophilized powder

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