Cholesterol Oxidase microbial, recombinant, expressed in E. coli, lyophilized powder, >=50 units/mg

Cholesterol Oxidase microbial, recombinant, expressed in E. coli, lyophilized powder, >=50 units/mg

Stock Code: 3579121
Manufacturer Part No: C1235-100UN
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Application


Cholesterol oxidase is used to determine serum cholesterol. The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3b-hydroxysteroids. Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures.


Biochem/physiol Actions


Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. This is subsequently isomerized to cholest-4-en-3-one with the release of H2O2. Optimum pH of the enzyme is 7.0. Hg2+, Ag+, ionic detergents inhibit the enzyme activity.


Packaging


100 units in glass bottle


Preparation Note


Dissolves in cold 50 mM potassium phosphate buffer, pH 7.0. Solution is to be prepared just before use.


Unit Definition


One unit will convert 1.0 µmol of cholesterol to 4-cholesten-3-one per minute at 37 °C and pH 7.0 in a peroxidase linked system.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Solubility50 mM potassium phosphate buffer, pH 7.0: soluble
Formlyophilized powder

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