Chitosanase from Streptomyces sp., buffered aqueous glycerol solution, >=15 units/mg protein (E1%)

Chitosanase from Streptomyces sp., buffered aqueous glycerol solution, >=15 units/mg protein (E1%)

Stock Code: 3579107
Manufacturer Part No: C0794-10UN
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Application


Chitosanase from Sigma has been used for polarized light-stimulated, enzymatic hydrolysis of chitin and chitosan.


Biochem/physiol Actions


Chitosanase catalyzes the endohydrolysis of β-1,4-linkages between D-glucosamine (GlcN-GlcN) residues in chitosan. The enzyme from Streptomyces has been reported to also hydrolyze the GlcNAc-GlcN linkage in partially acetylated chitosan.


Other Notes


View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer


Physical form


Solution in 50% glycerol containing 50 mM sodium acetate


Preparation Note


Soluble in 10mL of 0.1 M Acetate buffer at pH 5.5


Unit Definition


One unit will liberate 1.0 µmole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.5 at 37 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Solubility0.1 M acetate buffer, pH 5.5: soluble
Formbuffered aqueous glycerol solution

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