Apotryptophanase from Escherichia coli, soluble powder, 75-150 units/mg solid

Descriptions

Application

Apotryptophanase is used for the quantitative determination of pyridoxal-phosphate. Apotryptophanase, from Sigma, has been used to study pregnancy-associated PLP deficiency and vitamin B-6 deficiency .

Biochem/physiol Actions

Apotryptophanase hydrolizes tryptophan and is capable of catalyzing ?,?-elimination reactions with a number of substituted amino acids, including S-methyl-, S-ethyl- and S-benzyl- L-cysteine. DTNB inactivates tryptophanase .

Packaging

25 mg in poly bottle

Unit Definition

One unit releases one µg of indole from L-tryptophan in 10 min at pH 8.3 at 37 °C in the presence of 0.04 mM pyridoxal-5′--phosphate.