Pepsin from porcine gastric mucosa, Ph Eur

Pepsin from porcine gastric mucosa, Ph Eur

Stock Code: 3578882
Manufacturer Part No: 77161-100G
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Analysis Note


Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60?°C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.


Application


Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.


Biochem/physiol Actions


Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin


Other Notes


Sales restrictions may apply

ManufacturerSIGMA-ALDRICH
Agencytested according to Ph. Eur., USP/NF
Storage Temp.2-8°C
Formpowder

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