Pepsin from porcine gastric mucosa, powder, slightly beige, 600-1200 U/mg

Stock Code: 3578881
Manufacturer Part No: 77160-25G

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Descriptions

Analysis Note

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60?°C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.

Application

Pepsin cleavage can be used to produce F(ab′)₂ fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.

Biochem/physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Other Notes

Peptide synthesis, Review.

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Unit Definition

One unit corresponds to the amount of enzyme which increases the absorbance at 280 nm by 0.001 per minute at pH 2.0 and 37°C (Hemoglobin, Catalog No. 51290, as substrate); 15,000 absorbance units as described above are equivalent to ∼1 Bergmeyer unit. One Bergmeyer unit is the amount of enzyme which hydrolyzes 1 μmole Acetyl-L-phenyl-3,5-diiodo-L-tyrosine at pH 2.0 and 37°C.