Cellulase from Aspergillus niger, powder, off-white, approx. 0.8 U/mg

Cellulase from Aspergillus niger, powder, off-white, approx. 0.8 U/mg

Stock Code: 3578822
Manufacturer Part No: 22178-25G
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Application


Cellulase from Aspergillus niger has been used:in the preparation of the standardized solution to determine absolute cellulase levelsin phosphate-citric acid buffer for activity assayin non-starch polysaccharide degrading enzyme cocktail for in vitro hydrolysis of defatted rice bran and in the approach to improve the nutritive value of poultry feed


Biochem/physiol Actions


Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.


General description


Cellulase is a member of glycoside hydrolase family and is produced by a number of cellulolytic microbes.


Other Notes


View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer


Unit Definition


1 U corresponds to the amount of enzymes which liberates 1 µmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C

Colouroff-white
Quality Level100
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formpowder

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