HIV Protease Substrate 1,

Stock Code: 3578338
Manufacturer Part No: H6660-1MG

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Descriptions

Application

HIV Protease Substrate 1 has been used: to study protein structure-function relationships and in the fluorescent assayto evaluate protease inhibitors and for in vitro detection of the HIV-1 protease activityand in protease kinetics to study its enzymatic degradation rates

Biochem/physiol Actions

HIV Protease Substrate 1 is a synthetic peptide sequence that contains the cleavage site (Tyr-Pro) for the human immunodeficiency virus (HIV) Protease. It has two covalently modified amino acids for the detection of cleavage. One modification is the addition of the fluorophore 5-(2-aminoethylamino)-1-naphthalene sulfonate (EDANS) to the glutamic acid residue, while the other one includes the addition of the acceptor chromophore 4c-dimethylaminoazobenzene-4-carboxylate (DABCYL) to the lysine residue. The modified amino acids are on opposite sides of the cleavage site. Spatial orientation and overlap of the DABCYL absorbance with the EDANS emission permit resonance energy transfer between the two moieties. However, when the peptide is cleaved by the HIV protease, the DABCYL group is no longer proximal to the fluorophore.

Substrate for endopeptidases