Ala-Ala-Phe-7-amido-4-methylcoumarin, protease substrate

Descriptions

Application

Ala-Ala-Phe-7-amido-4-methylcoumarin has been used:in the preparation of the reaction mixture for enzymatic assaysto initiate theenzyme reaction in tripeptidyl peptidase-1 (TPP1) enzyme activity assayto initiate the assay reaction in lysosomal hydrolases activity assay

General description

Ala-Ala-Phe-7-amido-4-methylcoumarin is a fluorogenic substrate for the proteolytic activity. It is a positively charged substrate molecule.

Substrates

Substrate for chymotrypsin and tripeptidyl peptidaseII.

Specifications

Quality Level	400
InChI key	FVRLYIFIDKXFHU-UHFFFAOYSA-N
InChI	1S/C25H28N4O5/c1-14-11-22(30)34-21-13-18(9-10-19(14)21)28-25(33)20(12-17-7-5-4-6-8-17)29-24(32)16(3)27-23(31)15(2)26/h4-11,13,15-16,20H,12,26H2,1-3H3,(H,27,31)
Manufacturer	SIGMA-ALDRICH
Storage Temp.	2-8°C
Solubility	ethanol: 20 mg/mL, clear, colorless to light yellow
Form	powder
Assay	≥98% (TLC)