Ala-Ala-Phe-7-amido-4-methylcoumarin, protease substrate

Ala-Ala-Phe-7-amido-4-methylcoumarin, protease substrate

Stock Code: 3578064
Manufacturer Part No: A3401-25MG
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Application


Ala-Ala-Phe-7-amido-4-methylcoumarin has been used:in the preparation of the reaction mixture for enzymatic assaysto initiate theenzyme reaction in tripeptidyl peptidase-1 (TPP1) enzyme activity assayto initiate the assay reaction in lysosomal hydrolases activity assay


General description


Ala-Ala-Phe-7-amido-4-methylcoumarin is a fluorogenic substrate for the proteolytic activity. It is a positively charged substrate molecule.


Substrates


Substrate for chymotrypsin and tripeptidyl peptidaseII.

Quality Level400
InChI keyFVRLYIFIDKXFHU-UHFFFAOYSA-N
InChI1S/C25H28N4O5/c1-14-11-22(30)34-21-13-18(9-10-19(14)21)28-25(33)20(12-17-7-5-4-6-8-17)29-24(32)16(3)27-23(31)15(2)26/h4-11,13,15-16,20H,12,26H2,1-3H3,(H,27,31)
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Solubilityethanol: 20 mg/mL, clear, colorless to light yellow
Formpowder
Assay≥98% (TLC)

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