Protamine sulfate usp

Protamine sulfate usp

Stock Code: 3577869
Manufacturer Part No: P3369-10G
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£376.07 (exc VAT) per 10G
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Application


Protamine sulfate has been used in the transduction oflentiviruses into human embryonic 293T cellsshRNA into mesenchymal stem cells/marrow stromal cells (MSC)lenti Sca1-OCT4 plasmid into human cord blood hematopoietic progenitor CD34+ cells


Biochem/physiol Actions


Protamine facilitates direct transfection of microbial cells, and of human cells using cationic liposomes. Protamine effectively neutralizes the anticoagulation effects of heparin.


Alkaline protamine binds to negatively charged heparin. Use of protamine during cardiovascular surgery sometime triggers anaphylactic reactions.


General description


Protamine sulfate is a cationic polypeptide having a molecular weight of 5 kDa, isolated from salmon sperm.


Packaging


10, 100 g in glass bottle

Quality Level200
ManufacturerSIGMA-ALDRICH
Technique(s)transduction: suitable
Agencymeets USP testing specifications
Storage Temp.2-8°C
Formsolid
Assay≥92% (chromatography), 90.0-110.0% dry basis

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