Hirudin recombinant from yeast, lyophilized powder, white, >1500 U/vial

Hirudin recombinant from yeast, lyophilized powder, white, >1500 U/vial

Stock Code: 3577591
Manufacturer Part No: 94581-1EA
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Application


Recombinant hirudin from yeast has been used in mannan-binding lectin serine protease 2 (MASP2) activation assay. It has also been used in pre-treating the nanosheets in phosphate buffered saline (PBS) for platelet adhesion assay.


Biochem/physiol Actions


Recombinant hirudin appears to be antigenic. Independent studies have found that 50-70% of patients treated for several days with recombinant hirudin develop at least one hirudin antibody isotype. The antibodies reduce the anticoagulant effect of hirudin, but prolong its plasma half-life by reducing renal filtration.


The anticoagulant, hirudin, is the most potent natural inhibitor of both soluble and clot-bound thrombin. It binds thrombin with high affinity, covering more than 20% of its surface area and occluding both the active site and exosite I (fibrinogen and PAR recognition site). Hirudin blocks thrombus growth and platelet activation. It is not metabolized in the bloodstream of humans and is eliminated unchanged via kidney filtration.


Unit Definition


1 U corresponds to 1 ATU (antithrombin unit; 1 ATU is defined as the amount of inhibitor that neutralizes 1 NIH unit of thrombin).

Colourwhite
Quality Level100
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Solubility0.1 M Tris, 0.2 M NaCl, pH 8.4: 1 vial/mL, clear, colorless
Formlyophilized powder

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