Human Insulin ELISA Kit, for serum, plasma, cell culture supernatants and urine

Human Insulin ELISA Kit, for serum, plasma, cell culture supernatants and urine

Stock Code: 3577565
Manufacturer Part No: RAB0327-1KT
Order Now for 21 day delivery
Click Add to Quote for price and delivery
Quantity: - +

Application


For research use only. Not for use in diagnostic procedures.Human Insulin ELISA Kit has been used to determine human insulin and pro-insulin concentration in serum and HAMECs (human adipose microvascular endothelial cells) which were isolated from human visceral fat of nondiabetic patients.Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)


Human Insulin ELISA Kit has been used: to measure the blood levels of human insulin and proinsulin in the transgenic mice to measure serum insulin indaibetic rats to measure insulin in Multiple Sclerosis patients


Biochem/physiol Actions


Insulin is responsible for two types of actions, excitatory and inhibitory. In its excitatory role, it increases the uptake of glucose and lipid synthesis, and in its inhibitory role it inhibits glycogenolysis, gluconeogenesis, lipolysis, proteolysis and ketogenesis. Insulin plays a vital role in regulation of blood glucose level within the normal range. Abnormal insulin secretion leads to various disorders such as diabetes, hyperglycemia or hypoglycemia. Type I diabetes is a result of autoimmune destruction of ? cells of pancreas, which leads to depletion of insulin. Mutant INS-gene Induced Diabetes of Youth (MIDY) syndrome is an autosomal dominant disorder caused by missense mutations, which lead to aberrant proinsulin folding. Impaired glucose tolerance (IGT) or non-insulin-dependent diabetes mellitus (NIDDM) is caused by resistance to insulin-stimulated glucose uptake.


General description


The Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Insulin and Proinsulin in serum, plasma, cell culture supernatants and urine.


Immunogen


Recombinant Human Insulin


Other Notes


A sample Certificate of Analysis is available for this product.Please type the word sample in the text box provided for lot number.

ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C

There are no downloads for this product.