PEDF; HU; ELISA KIT - 96 WELL

PEDF; HU; ELISA KIT - 96 WELL

Stock Code: 3577492
Manufacturer Part No: CYT420
Order Now for 7 day delivery
Click Add to Quote for price and delivery
Quantity: - +

Application


Used to detect/quantify: PEDF


Research CategoryNeuroscience


Research Sub CategoryNeurochemistry & Neurotrophins


This ChemiKine Pigment Epithelium Derived Factor, Sandwich ELISA is used to measure & quantify PEDF levels in Neuroscience research.


ChemiKine™ PEDF kit is designed to measure the amount of PEDF in cell culture supernatants, tissue homogenates and biological fluid (vitreous, serum, plasma, and serum-free) samples of human origin. There are enough reagents included in this kit for one 96-well immunoassay plate. Running duplicate wells for samples and standards is recommended.

For Research Use Only. Not for use in diagnostic procedures.


Components


PEDF ELISA Plate: (Part No. 61597) One 96-Well Immunoplate Pre-coated with Mouse anti-Human PEDF Monoclonal Antibody, sealed in a foil pouch.Wash Buffer Concentrate: (Part No. 60245) One 100 mL (10X) bottle of Concentrate.Assay Diluent: (Part No. 60240) One 60 mL bottle (Ready to Use).PEDF Standard (Human): (Part No. 61600) One vial containing 250 ng recombinant full length human PEDF (Lyophilized).Biotinylated Mouse anti-Human PEDF Monoclonal Antibody: (Part No. 61598) One 50 μL vial.Streptavidin, HRP conjugated: (Part No. 61599) One 50 μL vial.TMB/E Solution: (Part No. 60096) One 10 mL bottle of a Ready to Use solution of 3,3′,5,5′-tetramethylbenzidine in a proprietary buffer with enhancer.Stop Solution (Part No. 60193): One 12 mL bottle of an HCl solution (Ready to Use).


Disclaimer


For research use only. Not for use in diagnostic procedures.


General description


Introduction

Pigment Epithelium-Derived Factor (PEDF) was first identified as a factor secreted by primary retinal pigment epithelial (RPE) cells that promotes the survival of neurons in vitro and in vivo. Molecular characterization of PEDF shows it to be a 50 kDa member of the serpin family of protease inhibitors1. A second mode of action of PEDF was identified with the demonstration that PEDF induces apoptosis of microvascular endothelial cells, a property that maintains the avascular nature of the retina2. As a number of ophthalmologic disorders are characterized by dysregulation of the retinal innervation and microvasculature, PEDF serves as an important regulator of human eye disease3. In addition, PEDF appears to have multifunctional antitumor activity in a neuroblastoma model, as Schwann cell-derived PEDF induces a differentiated, less malignant phenotype in neuroblastoma cells, promotes further growth and survival of Schwann cells, and inhibits angiogenesis4.

ChemiKine™ Pigment Epithelium-Derived Factor (PEDF) kit is a sandwich stripwell-type enzyme immunoassay (EIA), which measures PEDF. The kit will measure PEDF of vitreous fluid of human origin.

Test Principle

With the ChemiKine™ PEDF assay system, a mouse monoclonal antibody generated against human PEDF is coated onto a microplate and is used to capture PEDF from a sample. A second, biotinylated PEDF-specific mouse monoclonal antibody detects the captured PEDF. Peroxidase-labeled streptavidin detects the biotinylated mouse monoclonal antibody. After addition of the substrate and stop solution the amount of PEDF is determined

Quality Level100
ManufacturerSIGMA-ALDRICH

There are no downloads for this product.