DNMT3B/DNMT3L Active human, recombinant, expressed in baculovirus infected insect cells, >=90% (SDS-

DNMT3B/DNMT3L Active human, recombinant, expressed in baculovirus infected insect cells, >=90% (SDS-

Stock Code: 3576752
Manufacturer Part No: SRP0166-10UG
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Application


Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.


Biochem/physiol Actions


DNA (cytosine-5-)-methyltransferase 3 ? (DNMT3B) is a de novo methyltransferase which targets the un-methylated 5?-C-phosphate-G-3? (CpG) sites on the gene promoter. It is responsible for crucial epigenetic mechanisms for the regulation of chromosomal stability and for building a new methylation pattern. The protein is associated with promoter hypermethylation in tumorigenesis and lung cancer. DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is a non-catalytic accessory factor which is mainly responsible for the stimulation of DNA methylation. It helps the DNA methylation machinery to be positioned on properly chromatinized DNA regions. The protein also helps to provide uniform methylation patterns. It has an important role in spermatogenesis and mutations in the DNMT3L gene might be associated with azoospermia susceptibility.


General description


DNA (cytosine-5-)-methyltransferase 3 ? (DNMT3B) has two subfamilies-DNMT3B and ?DNMT3B. These are expressed in many transcript variants. Several single-nucleotide polymorphisms are observed within the promoter region of the DNMT3B gene. The gene encoding this protein is localized on human chromosome 20q11.2. DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is part of the DNA methyltransferases family. It has a cysteine-rich region containing a novel-type zinc finger domain. The gene encoding it is localized on human chromosome 21q22.3.


Physical form


Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCL, 20% glycerol, 4 mM glutathione.


Preparation Note


Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

ManufacturerSIGMA-ALDRICH
Formaqueous solution
Assay≥90% (SDS-PAGE)

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