LSD1 Active human, recombinant, expressed in E. coli, >=70% (SDS-PAGE)

LSD1 Active human, recombinant, expressed in E. coli, >=70% (SDS-PAGE)

Stock Code: 3576751
Manufacturer Part No: SRP0122-50UG
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Application


Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.


General description


Human LSD1, AOF2, amine oxidase, flavin containing, domain 2, GenBank Accession No. NM_015013, amino acids 158-end with N-terminal ST tag, MW = 103 kDa, expressed in E. coli expression system.


Physical form


Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.


Preparation Note


Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.


Unit Definition


One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37°C.

ManufacturerSIGMA-ALDRICH
Formaqueous solution
Assay≥70% (SDS-PAGE)

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