Protein Kinase A Catalytic Subunit from bovine heart, >=9 units/mug protein (cyclic-AMP is not requi

Protein Kinase A Catalytic Subunit from bovine heart, >=9 units/mug protein (cyclic-AMP is not requi

Stock Code: 3573528
Manufacturer Part No: P2645-400UN
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Application


Protein Kinase A (PKA) Catalytic Subunit from bovine heart has been used- to study PKA-mediated inhibition of IRK1 (inwardly rectifying K+) channels in in Vitro PKA pPhosphorylation assayin Vitro affinity binding assaysto study effects of PKA on inspiratory drive currents in functionally active motorneurons


Biochem/physiol Actions


Protein Kinase A catalyzes the transfer of terminal phosphate from ATP to threonine or serine residues present on various proteins. This protein is inactive in the absence of cAMP, where the catalytic and regulatory subunits are bound together. The regulatory subunit, in the presence of cAMP, binds to cAMP and releases the catalytic subunit.


Protein Kinase A (PKA) controls the transduction ofHedgehogsignaling and participates in proliferation and fate specification. It phosphorylates several neurotransmitter receptors, transcription factors and constituents of various intracellular signaling pathways.


General description


Protein Kinase A enzyme is composed of two subunits- catalytic and regulatory. The catalytic subunit exists as a monomer in the presence of cAMP and has a molecular weight of 40,862 Da.


Packaging


Package size based on phosphorylating units


Physical form


Lyophilized powder containing ≤ 1% protein with sucrose and phosphate buffer salts as stabilizer


Preparation Note


Prepared from protein kinase A (P 5511)


Unit Definition


One unit will transfer 1.0 picomole of phosphate from γ-32P-ATP to hydrolysed and partially dephosphorylated Casein per min at pH 6.5 at 30 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formlyophilized (white powder to sticky mass to hard pellet)

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