Xtt sodium salt bioreagent suitable for cell culture

Xtt sodium salt bioreagent suitable for cell culture

Stock Code: 3572774
Manufacturer Part No: X4626-100MG
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Application


XTT sodium salt has been used to assess cell viability.


XTT has been used in conjunction with phenazine methosulfate (PMS) to screen human cancer cell lines. The tetrazolium dye has also been used to study fungal cell damage, in testing antimicrobial susceptibility of staphylococci, and in Candida biofilm research. Limitations to the XTT assay have been reported.


Biochem/physiol Actions


XTT (sodium 3?-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is acted upon by dehydrogenases present in metabolically active cells. This produces water soluble formazan, which is a highly colored product. Thus, it offers an advantage in colorimetric proliferation assays, as opposed to MTT, as the product is already soluble and no formazan crystals are formed.


General description


XTT (sodium 3?-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is a tetrazolium salt, which forms water-soluble formazan on bioreduction.


Packaging


100, 500 mg in poly bottle


Principle


In living cells, XTT is metabolically reduced to produce a colorimetric, water-soluble formazan product.

Quality Level200
Pack100MG
InChI keyJACYMBNQPPWQML-UHFFFAOYSA-M
InChI1S/C22H17N7O13S2.Na/c1-41-17-8-15(28(31)32)19(43(35,36)37)10-13(17)26-24-21(22(30)23-12-6-4-3-5-7-12)25-27(26)14-11-20(44(38,39)40)16(29(33)34)9-18(14)42-2;/h3
ManufacturerSIGMA-ALDRICH
Product LineBioReagent
Formpowder
Assay≥90%

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