ESGRO Complete Accutase; 100ml

ESGRO Complete Accutase; 100ml

Stock Code: 3572618
Manufacturer Part No: SF006
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Application


Cell Detachment:

1. Thaw Accutase® to room temperature.

2. Wash plate, flask or beads with sterile PBS.

3. Add Accutase™ to culture dish or flask using aseptic procedures at 10 mL per 75 cm2 surface area.

4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).

5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required.


General description


ESGRO Complete™ Accutase™ is a cell detachment solution of proteolytic and collagenolytic enzymes that has been qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete™ Clonal Grade Medium (Cat. No. SF001-500). Accutase™ does not contain mammalian or bacterial derived products.


Legal Information


Accutase is a registered trademark of Innovative Cell Technologies, Inc.


CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


cOmplete is a trademark of Roche


Physical form


Frozen sterile liquid, ready to use formulation. Each lot is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.

1X Accutase® enzymes in Dulbecco′s PBS containing 0.5 mM EDTA 4Na and 3 mg/L Phenol Red.


Storage and Stability


Stable when stored at -20°C. Refer to lot expiration date on label. Recommended storage upon receipt is -20°C. After thawing, Accutase® may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMPERATURE.

Quality Level100
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - stem cell: suitable
Formliquid

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