BioTrac 530 Red Caspase-3 Dye (PBS)

BioTrac 530 Red Caspase-3 Dye (PBS)

Stock Code: 3572570
Manufacturer Part No: SCT105
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£675.54 (exc VAT) per EACH
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Application


Research Sub CategoryLive Cell Dye


Live cell imaging apoptosis dye for caspase-3/7 enzyme activity used to detect programmed cell death using fluorescence microscopy or flow cytometry applications.


Live cell fluorescent imaging


Research CategoryCell Imaging


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


Caspases are a family of protease enzymes that play essential roles in programmed cell death, termed apoptosis. Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9 and sequential activation of plays a central role in the execution-phase of cellular apoptosis.

The BioTracker NucView™ 530 Red Caspase-3 Dye provides a convenient live cell tool for profiling apoptotic cell populations based on caspase-3/7 activity using either fluorescence microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, the BioTracker NucView™ 530 Red Caspase-3 Dye can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with bright red fluorescence. Thus, NucView™ 530 Caspase-3 Substrate is bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. The dye is formaldehyde-fixable and compatible with subsequent immunostaining. The dye is offered in DMSO and PBS (phosphate-buffered saline) formulations. The substrate in PBS is formulated for use in cells that are sensitive to DMSO toxicity.

NucView™ 530 Caspase-3 Substrate stains apoptotic cell nuclei with orange fluorescence, for detection in the Cy™3 channel by fluorescence microscopy or the PE channel by flow cytometry. Note that when excited by the 488nm laser line, NucView™ 530 also fluoresces in the FITC channel, and therefore cannot be analyzed together with green probes by flow cytometry.


Legal Information


NucView is a registered trademark of Biotium Inc.


Physical form


Liquid


Quality


Absorbance Spectrum: 523 +/- 5
TLC: >95%


Storage and Stability


Store BioTracker NucView™ 530 Red Caspase-3 Dye, 1mM in PBS at -20°C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

ManufacturerSIGMA-ALDRICH
Technique(s)cell based assay: suitable

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