BioTracker Cystine-FITC Live Cell (1mg)

Stock Code: 3572544
Manufacturer Part No: SCT047
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Application


Research Sub CategoryLive Cell Dye


Live cell fluorescent imaging


Research CategoryCell Imaging


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


Uptake of cystine allows accumulation of cysteine that is necessary for glutathione synthesis and reactive oxygen species (ROS) detoxification. After the uptake of cystine into the cytoplasm, the reducing intracellular environment leads to a separation of one cystine molecule into two cysteine molecules. A key function of cysteine is to maintain the cellular pools of glutathione (GSH). Cystine uptake is also required for T-cell activation and B-cell development.The BioTracker Cystine-FITC dye is a live cell green fluorescent imaging probe that measures cystine uptake at the single cell level. The probe has been used to measure T-cell activation using traditional and imaging flow cytometry methods. Spectral PropertiesAbsorbance: 450nmEmission: 525nm


Physical form


Lyophilized


Quality


Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 625.65 g/mol


Storage and Stability


Store BioTracker Cystine-FITC Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

ManufacturerSIGMA-ALDRICH
Technique(s)cell based assay: suitable
Assay≥98% (elemental analysis), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (H-NMR)

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