Application
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
CFBE41o- 6.2 δF508-CFTR human CF bronchial epithelial cell line may be used to study the relationship between CFTR mRNA expression and Cl transport function.
Cell Line Description
Epithelial Cells
Disclaimer
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Cystic Fibrosis (CF) is a lethal autosomal recessive disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene which functions as a cAMP-activated and phosphorylated-regulated Cl channel. The predominant mutation in the CFTR gene is a trinucleotide deletion that results in loss of a phenylalanine at amino acids 508 (?F508) in the CFTR protein. This mutation accounts for ~66% of all CF alleles . CFBE41o- 6.2 ?F508-CFTR Human CF Bronchial Epithelial Cell line is a subclone derived from the electroporation of the parental CFBE41o- cell line with an Epstein-Barr virus (EBV)-based episomal pCEP4? vector containing the 6.2 kb full length ?F508 CFTR cDNA and a Hygromycin B resistance gene . The 6.2kb ?F508CFTR cDNA contains the trinucleotide TTT at the ?F508 locus rather than the naturally occurring CTT and thus makes it possible to differentiate between endogenous ?F508 CFTR and plasmid derived ?F508CFTR expression. The 6.2kb ?F508CFTR cDNA contains both the 5’; and 3’; UTR sequences that are known to affect translational efficiency and mRNA stability. The parental CFBE41o- is a CF human bronchial epithelial cell line, derived from a CF patient homozygous for the ?F508 CFTR mutation and immortalized with the origin-of-replication defective SV40 plasmid (pSVori-) . Established CF bronchial epithelial cell lines that are complemented with either wild-type or ?F508CFTR mRNA will help provide insights into the relationship between transgene-derived CFTR mRNA expression and rescue of cAMP-dependent Cl transport function.
Quality
Each vial contains ≥ 1X106 viable cells.
Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
Cells are negative for mycoplasma contamination.
